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KMID : 1036420160090010001
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대한치의학회 영문학회지
2016 Volume.9 No. 1 p.1 ~ p.8
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Evaluation of the Viability of Rat Periodontal Ligament Cells after Storing at 0℃/2 MPa Condition up to One Week - In Vivo MTT Method
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Jang Sun-Mi
Cho Sin-Yeon
Kim Eui-Seong
Jung Il-Young
Lee Seung-Jong
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Abstract
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Purpose: The aim of this study was to evaluate the rat periodontal ligament cell viability under 0℃/2 MPa condition up to one week using in vivo 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide (MTT) assay.
Materials and Methods: As soon as 110 upper molar teeth of rats were extracted, they were stored in Hartman’s solution under 0℃/2 MPa condition for 1, 2, 3, 4, and 7 days each. All specimens were treated with in vivo MTT assay and the value of optical density was measured by ELISA reader. These values were statistically analyzed by one-way ANOVA.
Result: There was no statistical difference on MTT value between immediate and 1 day storage group. There were statistically significant differences between 1 day and 2 days storage, 2 and 3 days storage groups, respectively. Teeth of 3, 4, and 7 days storage groups showed significantly lower MTT values compared with shorter period storage groups.
Conclusion: When the MTT values were substituted in standard curve, 1 day storage group at 0℃/2 MPa condition showed 68% cell viability when compared with immediate group. It dropped to 13% at 2 days, and to less than 5% at 3 days or more.
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KEYWORD
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MTT assay, Period, Periodontal ligament cell, Tooth storage
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